Journal: Molecular Metabolism
Article Title: TRPC6 governs brown adipose thermogenesis via a BMPR2-p38 MAPK signaling axis
doi: 10.1016/j.molmet.2026.102341
Figure Lengend Snippet: TRPC6 deficiency in BAT aggravates HFD-induced Obesity. ( A – H ) Trpc6 fl/fl and Trpc6 BTKO mice were fed normal chow diet (NCD) for 9 weeks. ( A ) Representative images of mice. ( B ) Body weight (n = 6). ( C ) Averaged weekly food intake (n = 9 weeks/cage). ( D ) Representative images of BAT. ( E ) Relative BAT weight (n = 6). ( F ) Representative H&E stains of BAT (Scale bar, 100 μm). ( G ) Lipid droplet area distribution and ( H ) means in BAT (n = 7–8). ( I – V ) Trpc6 fl/fl and Trpc6 BTKO mice were fed high-fat diet (HFD) for 14 weeks. ( I ) Representative appearance of mice. ( J ) Body weight (n = 6). ( K ) Body composition of mice (n = 6). ( L ) Representative images of BAT. ( M ) Relative weights of adipose tissue and liver (n = 7–10). ( N ) Representative H&E stains of BAT (Scale bar, 100 μm). ( O ) Lipid droplet area distribution and ( P ) means in BAT (n = 7–8). ( Q ) Representative H&E stains of liver (Scale bar, 200 μm). ( R , T ) Glucose tolerance test (GTT) and insulin tolerance test (ITT) (n = 6). ( S , U ) Analysis of the GTT and ITT data using an area of the curve (n = 6). Data are presented as mean ± SEM and ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, ns, not significant.
Article Snippet: The body composition of mice was measured using a small animal body composition analyzer (Niumag, QMR20-060H–I) as previously described [ ], which provided measurements of fat mass, lean mass, and free fluid.
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